Top attune flow cytometer Secrets
Top attune flow cytometer Secrets
Blog Article
Information-enriched characterization of mobile populations. Morphological facts from illustrations or photos can add into the richness of flow cytometry data. Such as, the determine exhibits an in any other case standard apoptosis assay utilizing Annexin V and PI, introducing mobile imaging to characterize cells in each populace to reveal morphologically distinctive functions. These insights could not happen to be obtained from multiplex staining by itself.
As samples are acquired to the Attune CytPix Flow Cytometer, the superior-velocity brightfield camera captures and stores photos of detected activities at a price around 6,000 visuals for each next, depending upon the flow charge and picture sizing. For increased adaptability, Attune Cytometric Computer software lets you adjust the image seize frequency as required and select a specific gate for image capture, if needed.
Incorporate acoustic focusing engineering with significant-parameter Examination for versatile spectral unmixing and standard workflows.
The award-successful Invitrogen Attune CytPix Flow Cytometer combines acoustic concentrating having a higher-speed brightfield digital camera for simultaneous large throughput flow cytometry and superior resolution brightfield imaging.
*Pricing is indicative only and is also subject matter to vary. Remember to Call your Agilent agent with any thoughts. The knowledge set forth herein just isn't a proposal or even a determination, each of which are topic to credit score acceptance, conditions and terms, and availability with Agilent. Agilent Economical Options is available in chosen countries.
In this article, a skilled user has gated singlets confidently. Immediately after assessing the handbook singlet gate, the CytPix picture-derived parameter ParticleCount reveals this gate includes more than 4% aggregates.
Pair your Attune flow cytometer having an autosampler for elevated workflow performance, and insert robotic integration for maximum throughput.
Our scientists will structure panels in your case as persistently you require—gratis, no product acquire needed.
The Attune here NxT cytometer will be the reliable workhorse instrument your lab can depend on for regular and trusted performance. The Attune CytPix product includes a superior-pace brightfield digital camera and AI-pushed picture Evaluation software program, with latest updates like device Understanding applications and an intuitive user interface, enabling Increased info precision, accuracy, and speedier insights into sample biology.
Most likely most importantly, these activities include cells of clearly diverse phenotypes which could lead on to incorrect conclusions relating to double beneficial activities (specifically in scarce populations).
Audio waves help exact cell alignment, causing Excellent knowledge high-quality and ~10X faster sample flow costs.
We Earlier demonstrated the strength of imaging Automobile-T/Ramos cell interactions. Enable’s have a look at just the inhabitants of finest curiosity, the double optimistic gatherings, to learn more. We could now use prolonged morphological parameters (in this article, circularity and skewness of intensity) to more study the attributes of such populations and refine gating on these activities, rising information robustness.
Leverage the strength of Sophisticated know-how and innovation to accelerate your discoveries. Have faith in while in the Attune Flow Cytometer family to provide the trustworthiness, velocity, and accuracy that set your perform aside.
No wash/no lyse leukocyte Investigation with imaging. Cells were being obtained from 24-hour-aged blood by dilution in one mM EDTA ( Expert UX/UI allows users to educate AI models for expanded purposes. (A) The “Prepare Model” feature presents annotation instruments and actual-time design instruction precision.
Gating on these extended parameters means that you can quickly and accurately identify populations of interest and ensure gating system with little or no handbook review. The Attune software package offers use of condition-of-the-art equipment Finding out analysis resources in an expertly designed user interface, making it exceptionally simple to use.
As the cell line had Formerly been analyzed over the Attune CytPix Flow Cytometer, the researchers went again to the photographs and have been capable to doc the microbial infection at the very least 5 times before.
Agilent Worth Guarantee ensures you at the very least 10 years of instrument use from your day you purchase. Otherwise, we will credit rating you With all the residual value of the method toward an upgraded model.
Listed here, we demonstrate that by gating approaches making use of picture-dependent quantitative parameters can distinguish interacting cells from coincident situations to additional precisely assess interacting cells.